ChromasPro - DNA测序和分析软件

ChromasPro用于将序列读数组装成contigs。有一个图形contig编辑器，显示对齐的色谱图。它还可以进行一般的序列分析，如限制性酶图谱、开放读码框搜索和BLAST提交。

ChromasPro适用于高达几兆的DNA序列组装项目，以及基本的序列编辑和分析。它能够组装来自Sanger测序仪（如ABI）和454和Illumina下一代测序仪的数据，如果有8GB内存的话，至多可组装1,000,000条序列。ChromasPro具有以下特点：

• 以Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF和纯文本格式打开序列。

• 以.ab1、.scf、.fasta和.fastq格式保存序列。

• 使用图形界面组装重叠的序列，生成共识，并突出显示模棱两可的地方供编辑。

• 使用质量数据自动去除低质量的序列，以提高序列组装。

• 使用参考序列作为组装的支架。

• 生成限制性位点和片段图，并列出切割、无切割和片段。

• 在G+C框架图的帮助下，绘制开放阅读框架图，并一键翻译ORFs。

• 打印色谱图、限制性位点和片段图以及开放阅读框架图。

• 通过NCBI网站进行核苷酸和蛋白质BLAST搜索。

• 通过与ClustalW对接进行多重比对。

• 反转和补充序列和色谱图。

• 通过准确匹配或optimal排列搜索序列。

• 在编辑核苷酸序列时显示翻译。

• 进行反向翻译并绘制核苷酸退化图。

• 绘制蛋白质的亲水性和抗原性。

• 复制色谱图部分的图像，以便粘贴到文档或演示文稿中。

系统要求：

与Windows 7sP1，8，10兼容

【英文介绍】

ChromasPro is for assembly of sequence reads into contigs, with a graphical contig editor which displays aligned chromatograms. It also peforms general sequence analysis such as restriction enzyme mapping, open-reading-frame searches, and BLAST submission.

ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features:

• Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.

• Save sequences in .ab1, .scf, .fasta and .fastq formats.

• Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.

• Use quality data to automatically remove low quality sequence for improved sequence assembly.

• Use a reference sequence as a scaffold for assembly.

• Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.

• Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.

• Print chromatograms, restriction site and fragment maps, and open reading frame maps.

• Perform nucleotide and protein BLAST searches through the NCBI web site.

• Perform multiple alignments by interfacing with ClustalW.

• Reverse & complement sequences and chromatograms.

• Search for sequences by exact matching or optimal alignment.

• Display translations when editing nucleotide sequences.

• Perform reverse translations and plot nucleotide degeneracy.

• Plot hydrophilicity and antigenicity of proteins.

• Copy an image of a chromatogram section for pasting into documents or presentations.