LAMP Designer - 引物数据分析软件

LAMP Designer可以设计用于环介导等温扩增测定的高效引物，在等温条件下扩增DNA和RNA序列，无需PCR设置。该技术依赖于自动循环和DNA聚合酶介导的链置换DNA合成。在不到一小时的时间内将几个DNA拷贝扩增到10⁹.逆转录偶联LAMP可用于RNA序列的扩增。

LAMP Designer特征：

设计用于环介导等温扩增的引物

LAMP采用四种专门设计的引物（两个内引物和两个外引物），可识别目标DNA中的六个不同区域。四种引物与目标DNA的杂交对于LAMP的效率来说是很关键的一步。因此，这四种引物的设计对于成功的测定很关键。

避免交叉同源

LAMP Designer会自动解释BLAST搜索结果并避开这些区域来设计与数据库具有显着交叉同源性的引物。

验证BLAST

可以针对数据库对引物进行BLAST搜索以验证它们的特异性。

多重LAMP引物组

可以检查为序列设计的引物的多重性。程序显示设计的引物之间稳定的交叉同源性的自由能。

导出结果

设计的引物组及其属性可以以标准csv或excel格式导出。

数据和数据库管理

可以创建多个项目。通过为每个实验创建单独的项目，可以轻松管理多个实验的数据。它维护一个用于序列信息和搜索结果的本地数据库。

系统要求：

Windows支持的平台： XP/Vista/Windows 7/Windows 8/Windows 10

【英文介绍】

Design Primers for Loop Mediated Isothermal Amplification

LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on auto-cycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 109 in less than an hour. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences.

Avoid Cross Homologies

LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database.

Verification BLAST

The primers can be BLAST searched against a database to verify their specificity.

Multiplex LAMP Primer Set

The primers designed for a sequence can be checked for multiplexing. The free energies of the most stable cross homologies between the primers designed, is displayed by the program.

Export Result

The designed primer sets, along with their properties, can be exported in standard csv or excel formats.

Data & Database Management

Multiple projects can be created. Data of multiple experiments can be easily managed by creating a separate project for each experiment. It maintains a local database for sequence information and search results.